Like mrna, both trna and rrna are transcripts of chromosomal dna . TRNA及rRNA同mRNA一樣,都是染色體DNA的轉(zhuǎn)錄產(chǎn)物。
Pcr analysis and southern blotting all confirmed that the ced - 9 gene has been integrated into the chromosomal dna of these transgenic plants 對(duì)所得到96株再生苗進(jìn)行pcr檢測(cè),結(jié)果表明,陽(yáng)性苗比例為34 。
S . tenebrarius chromosomal dna , partially digested with sau3al to yield fragments of 6 ~ 15kb , was ligated with vector pij702 提取黑暗鏈霉菌h6總dna ,經(jīng)sau3ai不完全酶切、凝膠電泳,收集6 15kb片段連接到載體pij702 。
It confirmed that ibdv vp2 gene was integrated into nuclear chromosomal dna by pcr . pcr positve plants were double checked for incorporation of the recombinant gene by southern blots 在轉(zhuǎn)基因組織生長(zhǎng)成植株的過(guò)程中, vp2基因隨著植物細(xì)胞的生長(zhǎng)而得到表達(dá)。
Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b . subtil is chromosomal dna ( from glyb to apre ) , and 27 % homology with bacillus sp 將此重組質(zhì)粒phchi轉(zhuǎn)化巨大芽抱桿菌b . megateriumap25 ,得到兩株轉(zhuǎn)化子,分別命名為p25113一9 , p25113一10 。
Bhattacharyya np , basu p , das m , et al negligible male gene flow across ethnic boundaries in india , revealed by analysis of y - chromosomal dna polymorphisms [ j ] . genome res . 1999 aug ; 9 ( 8 ) : 711 - 9 李冬娜、應(yīng)大君、區(qū)采瑩,等.中國(guó)海南島黎族人群y染色體上四個(gè)微衛(wèi)星基因座的多態(tài)性研究.中華醫(yī)學(xué)遺傳學(xué)雜志[ j ] . 2003 ; 1 : 1 - 3
A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127 . a group of constitutive - expression fusions with different fluorescent strength were obtained 將7653r的總dna經(jīng)sau3ai部分酶解并克隆到phn127上,獲得的融合子庫(kù),從中篩選到gfp組成型表達(dá)的具有調(diào)控活性的dna片段。
Four colonies of transformed e . coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained . the gene fragment in these isolates was identified by the methods of plasmid processing . dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b . subtilis from 2599451 to 2812870 was 85 % , and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b . subtilis ) in genebank 測(cè)序并序列比較結(jié)果表明該基因片段同已發(fā)表的枯草芽孢桿菌幾丁質(zhì)酶和內(nèi)切葡聚糖酶編碼幕因的克隆及重組芽抱桿菌的構(gòu)建glyb一apre之間的同源性是最高的,為35 % ;同bacz ’了了ussp . bp23ce1b 、 b . p朋刀us內(nèi)切葡聚糖酶和b . pol理vxap一1 , 4一內(nèi)切葡聚糖酶的編碼基因的同源性只有27 % 。
Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates . the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2 , w5 t1 and t7 . these pcr amplified fragments were cloned , and sequenced 首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘,然后用選擇性無(wú)氮培養(yǎng)基進(jìn)行初篩得到29株菌落形態(tài)不同的菌株;接著用固氮酶結(jié)構(gòu)基因nifh的特異性引物對(duì)這29株菌進(jìn)行pcr擴(kuò)增,結(jié)果表明其中7個(gè)菌株具有nifh基因,這7個(gè)菌株的編號(hào)依次為: c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
Strain pseudomonas psuedoalcaligenese ys1 was capable of producing phas containing monomer of hb and mcl has in certain medium . phacl and phac2 , two key polyhydroxyalkanoates polymerase genes of pha biosynthesis were amplified and cloned from chromosomal dna of pseudomonas psuedoalcaligenese ys1 using pcr 本研究利用聚合酶鏈?zhǔn)椒磻?yīng)( pcr )技術(shù),從p . psuedoalcaligeneseys1染色體dna中擴(kuò)增并克隆了調(diào)控短鏈與中鏈pha生物合成的兩個(gè)關(guān)鍵酶基因: phac1 、 phac2基因。